6DSZ

Crystal structure of DDB1 in complex with DET1- and DDB1-associated protein 1 (DDA1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	155
Detector technology	CCD
Collection date	2015-02-24
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	63.196, 219.175, 89.317
Unit cell angles	90.00, 89.98, 90.00

Refinement procedure

Resolution	47.796 - 3.093
R-factor	0.2501
Rwork	0.247
R-free	0.30710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b5m
RMSD bond length	0.004
RMSD bond angle	0.773
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.800	3.200
High resolution limit [Å]	3.093	3.100
Rmeas	0.151	0.151
Rpim	0.064	0.373
Number of reflections	40025	2174
<I/σ(I)>	10.29
Completeness [%]	89.0
Redundancy	2.7	2.7
CC(1/2)	0.990	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M MES monohydrate, pH 6.5, 20% (w/v) PEG4000, 180 mM NaCl, 5 mM DTT