6DPL

Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 10 mM Mg2+ and 200 mM Rb+ for 720 s at 21 C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	81.856, 37.521, 62.706
Unit cell angles	90.00, 97.31, 90.00

Refinement procedure

Resolution	19.495 - 1.450
R-factor	0.152
Rwork	0.151
R-free	0.17920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 1ZBL
RMSD bond length	0.007
RMSD bond angle	1.024
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.500	19.500	1.470
High resolution limit [Å]	1.450	7.940	1.450
Rmerge	0.030	0.016	0.778
Rmeas	0.043	0.023	1.100
Rpim	0.030	0.016	0.778
Total number of observations	64252
Number of reflections	33423	216	1429
<I/σ(I)>	13.1
Completeness [%]	98.9	93.3	88.5
Redundancy	1.9	1.8	1.8
CC(1/2)	0.999	0.999	0.371

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2