6DPL
Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 10 mM Mg2+ and 200 mM Rb+ for 720 s at 21 C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.856, 37.521, 62.706 |
| Unit cell angles | 90.00, 97.31, 90.00 |
Refinement procedure
| Resolution | 19.495 - 1.450 |
| R-factor | 0.152 |
| Rwork | 0.151 |
| R-free | 0.17920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 1ZBL |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.024 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.500 | 19.500 | 1.470 |
| High resolution limit [Å] | 1.450 | 7.940 | 1.450 |
| Rmerge | 0.030 | 0.016 | 0.778 |
| Rmeas | 0.043 | 0.023 | 1.100 |
| Rpim | 0.030 | 0.016 | 0.778 |
| Total number of observations | 64252 | ||
| Number of reflections | 33423 | 216 | 1429 |
| <I/σ(I)> | 13.1 | ||
| Completeness [%] | 98.9 | 93.3 | 88.5 |
| Redundancy | 1.9 | 1.8 | 1.8 |
| CC(1/2) | 0.999 | 0.999 | 0.371 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2 |
