6DOW

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 160 s at 21 C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8
Spacegroup name	C 1 2 1
Unit cell lengths	82.035, 37.485, 62.504
Unit cell angles	90.00, 96.84, 90.00

Refinement procedure

Resolution	19.400 - 1.495
R-factor	0.166
Rwork	0.164
R-free	0.19810
Starting model (for MR)	pdbid 1ZBL
RMSD bond length	0.008
RMSD bond angle	1.032
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.400	19.400	1.520
High resolution limit [Å]	1.490	8.190	1.490
Rmerge	0.030	0.029	1.276
Rmeas	0.042	0.041	1.805
Rpim	0.030	0.029	1.276
Total number of observations	58729
Number of reflections	30351	190	1109
<I/σ(I)>	8.9
Completeness [%]	98.6	91.5	73.4
Redundancy	1.9	1.8	1.9
CC(1/2)	0.999	0.989	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2