6DOI

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid (1.54 Angstrom wavelength): Soak in 0.5 mM EGTA and 200 mM K+ at 21 C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-20
Detector	RIGAKU SATURN A200
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	81.456, 38.020, 62.071
Unit cell angles	90.00, 96.22, 90.00

Refinement procedure

Resolution	22.010 - 1.948
R-factor	0.1494
Rwork	0.147
R-free	0.19530
Starting model (for MR)	PDBID 1ZBL
RMSD bond length	0.009
RMSD bond angle	1.037
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.010	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.070	0.215
Number of reflections	12950
<I/σ(I)>	14.1	3.6
Completeness [%]	92.3	78.1
Redundancy	3.1	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2