6DO5

KLHDC2 ubiquitin ligase in complex with USP1 C-end degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	44.496, 87.086, 88.322
Unit cell angles	90.00, 104.56, 90.00

Refinement procedure

Resolution	43.067 - 2.500
R-factor	0.2029
Rwork	0.200
R-free	0.25250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vpj
RMSD bond length	0.013
RMSD bond angle	1.303
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.119	0.055	0.527
Rmeas	0.130	0.060	0.604
Rpim	0.053	0.024	0.287
Number of reflections	32634	1732	1338
<I/σ(I)>	6.6
Completeness [%]	98.0	99.9	80.3
Redundancy	5.9	6.1	3.8
CC(1/2)		0.997	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.03M MgCl2*6H2O, 0.03M CaCl2*2H2O, 0.05M Imidazole, 0.05M MES monohydrate, 12.5% v/v MPD, 12.5% PEG1000, 12.5% w/v PEG3350, pH 6.5.