6DO4

KLHDC2 ubiquitin ligase in complex with SelS C-end degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	44.637, 88.596, 88.824
Unit cell angles	90.00, 104.77, 90.00

Refinement procedure

Resolution	39.370 - 2.200
R-factor	0.1697
Rwork	0.167
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vpj
RMSD bond length	0.007
RMSD bond angle	0.894
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.138	0.057	0.592
Rmeas	0.156	0.064	0.702
Rpim	0.073	0.030	0.369
Number of reflections	33761	1755	1576
<I/σ(I)>	5.9
Completeness [%]	99.3	99.8	93
Redundancy	4.5	4.7	3.3
CC(1/2)		0.996	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.03M MgCl2*6H2O, 0.03M CaCl2*2H2O, 0.05M Imidazole, 0.05M MES monohydrate, 13.25% v/v MPD, 13.25% PEG1000, 13.25% w/v PEG3350, pH 6.8.