6DNZ
Trypanosoma brucei PRMT1 enzyme-prozyme heterotetrameric complex with AdoHcy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 196.582, 65.904, 141.028 |
| Unit cell angles | 90.00, 106.90, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.384 |
| R-factor | 0.1887 |
| Rwork | 0.188 |
| R-free | 0.22170 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.522 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((dev_3071: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.384 | 2.384 |
| Rmerge | 0.103 | 0.773 |
| Number of reflections | 97551 | 3757 |
| <I/σ(I)> | 22.2 | |
| Completeness [%] | 88.7 | 54 |
| Redundancy | 10.5 | 3.5 |
| CC(1/2) | 0.997 | 0.651 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | 7% (w/v) PEG 4000, 0.1 M Tris-HCl, pH 7.4 |
