6DLG

Crystal structure of a SHIP1 surface entropy reduction mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03319
Spacegroup name	P 21 21 21
Unit cell lengths	45.098, 73.200, 124.207
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.396 - 1.499
R-factor	0.1792
Rwork	0.178
R-free	0.20040
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS (November 3, 2014)
Data scaling software	XDS (November 3, 2014)
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.590
High resolution limit [Å]	1.499	1.500
Rmeas	0.059	0.787
Number of reflections	66070	10528
<I/σ(I)>	13.79	1.85
Completeness [%]	98.9	98.6
Redundancy	3.28	3.29
CC(1/2)	0.999	0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	295	0.1M HEPES-NaOH pH6.7, 20% PEG 1500, 5mM magnesium chloride