6DL7

Human mitochondrial ClpP in complex with ONC201 (TIC10)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	C 1 2 1
Unit cell lengths	142.390, 153.440, 104.790
Unit cell angles	90.00, 117.58, 90.00

Refinement procedure

Resolution	49.330 - 2.000
R-factor	0.2278
Rwork	0.226
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.568
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.330	2.071
High resolution limit [Å]	2.000	2.000
Number of reflections	133979
<I/σ(I)>	5.64
Completeness [%]	100.0
Redundancy	4.7
CC(1/2)	0.504

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	277	5%(w/v) PEG 4,000, 100mM KCl, 100mM NaAc (pH5.2)