6DKJ

human GIPR ECD and Fab complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	90
Detector technology	PIXEL
Collection date	2015-09-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	55.907, 63.649, 159.099
Unit cell angles	90.00, 92.56, 90.00

Refinement procedure

Resolution	19.719 - 1.950
R-factor	0.2005
Rwork	0.199
R-free	0.23840
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless (0.5.12)
Phasing software	PHASER
Refinement software	PHENIX ((dev-2328_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.740	19.740	1.990
High resolution limit [Å]	1.950	9.940	1.950
Rmerge	0.095	0.028	0.633
Rmeas	0.111	0.033	0.737
Rpim	0.057	0.017	0.375
Total number of observations	294866
Number of reflections	79376	563	4537
<I/σ(I)>	9.7
Completeness [%]	97.6	86.3	97.1
Redundancy	3.7	3.6	3.8
CC(1/2)	0.997	0.999	0.817

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10% PEG 4000 and 20% isopropanol