6DJL
Crystal structure of the Rab11 GEF SH3BP5 bound to nucleotide free Rab11A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 114.850, 197.040, 304.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.612 - 3.100 |
| R-factor | 0.237315729513 |
| Rwork | 0.235 |
| R-free | 0.27764 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.547 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CRANK2 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.136 | 2.189 |
| Rmeas | 0.153 | |
| Rpim | 0.068 | 1.072 |
| Number of reflections | 62902 | 6236 |
| <I/σ(I)> | 8.26 | 0.75 |
| Completeness [%] | 99.7 | 99.79 |
| Redundancy | 4.9 | 5.1 |
| CC(1/2) | 0.997 | 0.281 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 23% PEG-3350, 200 mM NaCl, 100 mM Bis Tris pH-6.5 |
