6DGP
Crystal Structure of Human PPARgamma Ligand Binding Domain in Complex with TRAP220 Coactivator Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-04-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.090, 54.730, 123.471 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 44.045 - 3.100 |
| R-factor | 0.2293 |
| Rwork | 0.222 |
| R-free | 0.29390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1prg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.282 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.040 | 3.211 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.366 | |
| Rmeas | 0.518 | |
| Number of reflections | 10895 | 1082 |
| <I/σ(I)> | 11.31 | 2.05 |
| Completeness [%] | 99.8 | 99.18 |
| Redundancy | 2 | 1.9 |
| CC(1/2) | 0.998 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M Magnesium acetate, 0.1M Sodium cacodylate, pH 6.5 15% w/v, PEG 6000 |
