6DGF

Ubiquitin Variant bound to USP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93.15
Detector technology	CCD
Collection date	2016-07-14
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	159.978, 58.093, 46.842
Unit cell angles	90.00, 98.12, 90.00

Refinement procedure

Resolution	39.069 - 2.340
R-factor	0.1782
Rwork	0.176
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq 2hd5
RMSD bond length	0.002
RMSD bond angle	0.459
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.340	2.340
Number of reflections	18044	1628
<I/σ(I)>	31.4	8.6
Completeness [%]	99.0	90.8
Redundancy	3.2
CC(1/2)	0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1 M MES buffer pH 6, 12% (w/v) PEG3350, 0.2M sodium sulfate