6DGF

Ubiquitin Variant bound to USP2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin carboxyl-terminal hydrolase 2	polymer	370	42467.0	1	UniProt (O75604) Pfam (PF00443)	Homo sapiens (Human)	41 kDa ubiquitin-specific protease,Deubiquitinating enzyme 2,Ubiquitin thioesterase 2,Ubiquitin-specific-processing protease 2
2	B (B)	Polyubiquitin-B	polymer	93	10611.2	1	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (Human)
3	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	D, E (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
5	F, G (A, B)	water	water		18.0	156	Chemie (HOH)

Sequence modifications

A: 250 - 605 (UniProt: O75604)

PDB	External Database	Details
His 236	-	expression tag
His 237	-	expression tag
His 238	-	expression tag
His 239	-	expression tag
His 240	-	expression tag
His 241	-	expression tag
Ser 242	-	expression tag
Ser 243	-	expression tag
Gly 244	-	expression tag
Leu 245	-	expression tag
Val 246	-	expression tag
Pro 247	-	expression tag
Arg 248	-	expression tag
Gly 249	-	expression tag

B: 1 - 74 (UniProt: P0CG47)

PDB	External Database	Details
Met -18	-	expression tag
Ala -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Val -10	-	expression tag
Thr -9	-	expression tag
Ser -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Lys -4	-	expression tag
Ala -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Thr 0	-	expression tag
Cys 2	Gln 154	engineered mutation
Cys 6	Lys 158	engineered mutation
Arg 11	Lys 163	engineered mutation
Cys 12	Thr 164	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	53078.2
Non-Polymers*	Number of molecules	3
	Total formula weight	257.5
All*	Total formula weight	53335.7

*Water molecules are not included.