6DF8

Kaiso (ZBTB33) zinc finger DNA binding domain in complex with the specific Kaiso binding sequence (KBS), pH 6.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03316
Spacegroup name	C 2 2 2
Unit cell lengths	44.371, 184.267, 104.938
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.138 - 2.536
R-factor	0.1838
Rwork	0.179
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f6m
RMSD bond length	0.011
RMSD bond angle	1.214
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.138	2.630
High resolution limit [Å]	2.536	2.540
Rmerge	0.117
Rmeas	0.127
Number of reflections	14573	1268
<I/σ(I)>	10.94
Completeness [%]	98.7	87.27
Redundancy	6.6	5.3
CC(1/2)	0.998	0.694

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.2 M Disodium tartrate, 20 % PEG 3350