6DF3

Crystal structure of ternary complex of IL-24 with soluble receptors IL-22RA and IL-20RB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 43
Unit cell lengths	77.702, 77.702, 124.785
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.150
R-factor	0.1838
Rwork	0.182
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4doh
RMSD bond length	0.019
RMSD bond angle	2.018
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.072	1.416
Rpim	0.034	0.620
Number of reflections	39903	3987
<I/σ(I)>	29.7	1.62
Completeness [%]	99.7	100
Redundancy	8	7.1
CC(1/2)	0.970	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	8 mg/mL protein in 0.2 M sodium chloride, 0.05 M HEPES against 215 w/v MEPEG2000, 0.05 M Tris, pH 8.5, 0.05 M trimethylamine N-oxide dihydrate