6DEC

Crystal structure of Bos taurus Arp2/3 complex binding with C-terminus of Homo sapiens SPIN90

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791829
Spacegroup name	P 21 21 21
Unit cell lengths	179.840, 197.381, 202.067
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.939 - 4.600
R-factor	0.2767
Rwork	0.275
R-free	0.31410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jd2
RMSD bond length	0.002
RMSD bond angle	0.478
Data reduction software	HKL-3000 (2.3.6)
Data scaling software	HKL-3000 (2.3.6)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.680
High resolution limit [Å]	4.600	4.600
Rpim	0.073	0.671
Number of reflections	39413	1921
<I/σ(I)>	11.8	1.5
Completeness [%]	93.9	93.2
Redundancy	5.4	4.7
CC(1/2)		0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	50 mM HEPES, pH 7.5, 5% PEG3350, 50 mM L-Proline, 0.5 mM ATP, 0.5 mM calcium chloride, 1 mM DTT