6DD5
Crystal Structure of the Cas6 Domain of Marinomonas mediterranea MMB-1 Cas6-RT-Cas1 Fusion Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9765 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 92.278, 110.760, 192.906 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.226 - 2.850 |
R-factor | 0.1888 |
Rwork | 0.187 |
R-free | 0.22520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b3w |
RMSD bond length | 0.006 |
RMSD bond angle | 0.772 |
Data reduction software | XDS (Jun 17, 2015) |
Data scaling software | Aimless (0.5.17) |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.230 | 48.230 | 2.950 |
High resolution limit [Å] | 2.850 | 11.040 | 2.850 |
Rmerge | 0.195 | 0.027 | 1.463 |
Rmeas | 0.202 | 0.028 | 1.514 |
Rpim | 0.052 | 0.008 | 0.389 |
Total number of observations | 696144 | ||
Number of reflections | 47006 | 915 | 4531 |
<I/σ(I)> | 17.3 | ||
Completeness [%] | 100.0 | 99.1 | 100 |
Redundancy | 14.8 | 13.1 | 15.1 |
CC(1/2) | 0.997 | 1.000 | 0.766 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 295 | 0.1 M Sodium Acetate, 2.5 M Ammonium Sulfate |