6DCW

Crystal structure of human anti-tau antibody CBTAU-27.1 Fab in complex with a human tau peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	92.308, 59.670, 82.331
Unit cell angles	90.00, 103.62, 90.00

Refinement procedure

Resolution	43.771 - 2.000
R-factor	0.1705
Rwork	0.168
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3QOS
RMSD bond length	0.005
RMSD bond angle	0.937
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	2.000	2.000
Rpim	0.060	0.250
Number of reflections	27347
<I/σ(I)>	19.5	3.5
Completeness [%]	91.5
Redundancy	4
CC(1/2)	0.990	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Hepes, pH 7.0, 0.1 M KCl, 15% PEG5000 MME.