6DCN

Bcl-xL complex with Beclin 1 BH3 domain T108pThr

Replaces:  5VB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-09
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9537
Spacegroup name	P 4 21 2
Unit cell lengths	109.419, 109.419, 97.127
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.934 - 2.444
R-factor	0.1923
Rwork	0.189
R-free	0.22860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2p1l
RMSD bond length	0.007
RMSD bond angle	0.796
Data reduction software	XDS (vNov 11, 2017)
Data scaling software	XDS (vNov 11, 2017)
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.934	2.505
High resolution limit [Å]	2.444	2.444
Number of reflections	22390
<I/σ(I)>	11.13
Completeness [%]	99.7
Redundancy	10.8
CC(1/2)	0.998	0.533

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2M NaCl, 10% glycerol