6DCL

Crystal structure of UP1 bound to pri-miRNA-18a terminal loop

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.8726
Spacegroup name	P 62 2 2
Unit cell lengths	127.028, 127.028, 147.064
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.066 - 2.497
R-factor	0.2032
Rwork	0.202
R-free	0.22810
RMSD bond length	0.008
RMSD bond angle	1.072
Data reduction software	XDS
Data scaling software	Aimless (0.5.9)
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.070	2.600
High resolution limit [Å]	2.497	2.500
Rmerge	0.089	0.953
Rmeas	0.094	1.008
Rpim	0.028	0.316
Number of reflections	24934	2731
<I/σ(I)>	19.7
Completeness [%]	99.9	99.2
Redundancy	9.8	9.2
CC(1/2)	0.999	0.801

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium citrate, 20% PEG 3350