6DC3
RSV prefusion F bound to RSD5 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | I 2 3 |
Unit cell lengths | 270.320, 270.320, 270.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.230 | 38.230 | 3.640 |
High resolution limit [Å] | 3.500 | 12.620 | 3.500 |
Rmerge | 0.104 | 0.035 | 0.846 |
Rmeas | 0.117 | 0.040 | 0.960 |
Rpim | 0.053 | 0.019 | 0.448 |
Total number of observations | 198500 | 3891 | 21266 |
Number of reflections | 41327 | 906 | 4656 |
<I/σ(I)> | 9.7 | 23.5 | 1.8 |
Completeness [%] | 99.9 | 95.5 | 100 |
Redundancy | 4.8 | 4.3 | 4.6 |
CC(1/2) | 0.995 | 0.997 | 0.528 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.8 M Lithium sulfate, 0.1 M Tris pH 8.0 |