6DBZ

Crystal structure of Nudix 1 from Arabidopsis thaliana complexed with isopentenyl diphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-01-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	36.050, 72.751, 116.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.691 - 1.900
R-factor	0.1891
Rwork	0.187
R-free	0.22920
RMSD bond length	0.013
RMSD bond angle	1.117
Data reduction software	MOSFLM (7.1.0)
Data scaling software	Aimless (0.3.11)
Phasing software	MOLREP (11.2.08)
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.750	9.130
High resolution limit [Å]	1.670	1.670
Rmerge	0.100
Number of reflections	36756
<I/σ(I)>	12.7
Completeness [%]	100.0
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	0.1 M succinic acid pH 5.0 20% PEG 4000 0.3 M magnesium nitrate