6DAD
1.65 Angstrom crystal structure of the N97I Ca/CaM:CaV1.2 IQ domain complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.273, 69.045, 85.792 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.511 - 1.650 |
R-factor | 0.1818 |
Rwork | 0.180 |
R-free | 0.20840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dae |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.511 | 41.511 | 1.750 |
High resolution limit [Å] | 1.650 | 4.930 | 1.650 |
Rmeas | 0.057 | 0.601 | |
Number of reflections | 90091 | 14567 | |
<I/σ(I)> | 14.82 | 2.17 | |
Completeness [%] | 99.9 | 99.7 | |
Redundancy | 3.92 | ||
CC(1/2) | 0.999 | 0.804 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2.1 M sodium malonate, 0.1 M HEPES, pH 7.5 |