6D8P

Ternary RsAgo Complex Containing Guide RNA Paired with Target DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	68.234, 119.216, 117.648
Unit cell angles	90.00, 95.62, 90.00

Refinement procedure

Resolution	42.336 - 2.100
R-factor	0.1843
Rwork	0.182
R-free	0.23480
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	101956
<I/σ(I)>	27.1
Completeness [%]	93.5
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	40% MPD, 30 mM magnesium acetate, 50 mM sodium cacodylate pH 5.7