6D4F

Crystal structure of PTP epsilon D2 domain (A455N/V457Y/E597D)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2015-01-13
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	61.470, 73.489, 75.656
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.015 - 1.909
R-factor	0.1926
Rwork	0.190
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jjd
RMSD bond length	0.007
RMSD bond angle	0.811
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2376: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.940
High resolution limit [Å]	1.909	1.910
Rmerge	0.064	0.486
Number of reflections	26610
<I/σ(I)>	28
Completeness [%]	97.4	71.8
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	292	0.1 M imidazole pH 8.0, 0.2 M calcium acetate hydrate, 20% (w/v) polyethylene glycol 1000