6D36
Structure of human ARH3 bound to ADP-ribose and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.918, 72.330, 115.945 |
| Unit cell angles | 83.06, 85.74, 72.04 |
Refinement procedure
| Resolution | 46.451 - 1.700 |
| R-factor | 0.1853 |
| Rwork | 0.184 |
| R-free | 0.21780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2foz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.954 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.451 | 1.719 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 146249 | |
| <I/σ(I)> | 21.9 | |
| Completeness [%] | 96.6 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 22% PEG4000, 0.1 M sodium acetate pH 4.5, and 0.1 M MgSO4 |
