6D30
Structure of human Usb1 with uridine-uridine, inactive H208Q mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-04-25 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9787 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.698, 53.038, 45.814 |
Unit cell angles | 90.00, 105.72, 90.00 |
Refinement procedure
Resolution | 44.100 - 1.170 |
R-factor | 0.1503 |
Rwork | 0.149 |
R-free | 0.17170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5v1m |
RMSD bond length | 0.014 |
RMSD bond angle | 1.297 |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.040 | 1.190 |
High resolution limit [Å] | 1.170 | 1.170 |
Number of reflections | 60998 | |
<I/σ(I)> | 13.7 | |
Completeness [%] | 94.1 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 100 mM Tris, 25% SOKALN CP 42, 10% tetrahydrofuran |