6D2Q
Crystal structure of the FERM domain of zebrafish FARP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-13 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97926 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.031, 59.436, 97.462 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.770 - 2.990 |
R-factor | 0.23 |
Rwork | 0.224 |
R-free | 0.28270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6d2k |
RMSD bond length | 0.002 |
RMSD bond angle | 0.471 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.770 |
High resolution limit [Å] | 2.990 |
Number of reflections | 6910 |
<I/σ(I)> | 12.75 |
Completeness [%] | 99.3 |
Redundancy | 11.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 0.1 M Bicine pH 9.0, 16 % MPD (v/v) |