6D2L

Crystal structure of human CARM1 with (S)-SKI-72

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	75.601, 155.638, 95.338
Unit cell angles	90.00, 101.04, 90.00

Refinement procedure

Resolution	50.010 - 2.000
R-factor	0.1874
Rwork	0.187
R-free	0.23580
RMSD bond length	0.008
RMSD bond angle	1.273
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0218)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.155	0.122	0.916
Rmeas	0.176	0.136	1.048
Rpim	0.081	0.061	0.501
Total number of observations	640160
Number of reflections	142452	7129	7016
<I/σ(I)>	4.1
Completeness [%]	97.0	95.4	96.2
Redundancy	4.5	4.8	4.1
CC(1/2)		0.975	0.543

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	25% PEG 3350, 0.2 M NH4SO4, 0.1 M HEPES pH7.5