6D1V
Crystal structure of E. coli RppH-DapF complex, monomer bound to RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97910 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 160.644, 191.986, 51.207 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.601 - 1.810 |
R-factor | 0.1994 |
Rwork | 0.199 |
R-free | 0.21960 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.036 |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 123.200 | 1.850 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.037 | 0.989 |
Number of reflections | 70344 | |
<I/σ(I)> | 12.6 | |
Completeness [%] | 96.8 | |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 292 | 30% (v/v) PEG400 and 0.1 M CHES, pH 9.2 |