6D1U
Crystal structure of the human CLR:RAMP1 extracellular domain heterodimer in complex with adrenomedullin 2/intermedin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-05 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.295, 106.041, 136.911 |
| Unit cell angles | 90.00, 122.53, 90.00 |
Refinement procedure
| Resolution | 45.640 - 2.050 |
| R-factor | 0.1954 |
| Rwork | 0.194 |
| R-free | 0.22280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rwg |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.519 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 5.560 | 2.050 |
| Rmerge | 0.062 | 0.027 | 1.202 |
| Rmeas | 0.067 | 0.029 | 1.296 |
| Rpim | 0.024 | 0.011 | 0.480 |
| Total number of observations | 965268 | ||
| Number of reflections | 128678 | 6609 | 5887 |
| <I/σ(I)> | 8.5 | ||
| Completeness [%] | 98.9 | 99.2 | 91 |
| Redundancy | 7.5 | 7.3 | 6.5 |
| CC(1/2) | 0.999 | 0.759 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 13% v/v PEG 3,350 0.1 M Na cacodylate, pH 6.5 0.15 M DL-malic acid |
