6D12

Crystal structure of C-terminal xRRM domain of human Larp7 bound to 7SK stem-loop 4 RNA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979300
Spacegroup name	P 1 21 1
Unit cell lengths	47.910, 34.030, 95.010
Unit cell angles	90.00, 95.18, 90.00

Refinement procedure

Resolution	94.622 - 2.205
R-factor	0.2299
Rwork	0.228
R-free	0.26730
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	0.999
Data reduction software	XDS (20170615)
Data scaling software	XSCALE (20170615)
Phasing software	HKL2Map (2016/1)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	94.622	94.622	2.260
High resolution limit [Å]	2.200	9.830	2.200
Rmerge	0.169	0.091	1.191
Rmeas	0.181	0.098	1.279
Total number of observations	120222
Number of reflections	15929	207	1187
<I/σ(I)>	6.54	17.27	1.43
Completeness [%]	99.9	99.5	99.9
Redundancy	7.547	6.604	7.48
CC(1/2)	0.996	0.998	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	293	0.05M Na Cacodylate,0.08M Mg acetate, 24% PEG 4000