6CZZ
Crystal structure of Arabidopsis thaliana phosphoserine aminotransferase isoform 1 (AtPSAT1) in complex with PLP-phosphoserine geminal diamine intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 189.668, 53.288, 137.921 |
| Unit cell angles | 90.00, 91.91, 90.00 |
Refinement procedure
| Resolution | 44.400 - 1.700 |
| R-factor | 0.1812 |
| Rwork | 0.181 |
| R-free | 0.20870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xk1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.305 |
| Data reduction software | XDS (May 1, 2016) |
| Data scaling software | XDS (May 1, 2016) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.400 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.080 | 0.554 |
| Number of reflections | 149284 | 23323 |
| <I/σ(I)> | 10.09 | 1.83 |
| Completeness [%] | 98.1 | 95.6 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 0.2 M lithium sulfate, 17% PEG 3350 and 0.1 M Tris at pH 8.5 |
