6CZW
Crystal structure of PT1940 bound to HIF2a-B*:ARNT-B* complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.928, 83.534, 41.525 |
| Unit cell angles | 90.00, 106.72, 90.00 |
Refinement procedure
| Resolution | 27.010 - 1.600 |
| R-factor | 0.2134 |
| Rwork | 0.211 |
| R-free | 0.25050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xt2 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.369 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmeas | 0.079 | |
| Rpim | 0.041 | 0.558 |
| Number of reflections | 31368 | 2985 |
| <I/σ(I)> | 25 | 1.1 |
| Completeness [%] | 98.9 | 95.2 |
| Redundancy | 3.53 | 3 |
| CC(1/2) | 0.504 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 50 mM Bis-Tris pH5.4, 16% PEG 3350. Seed with crushed crystal right after drops are set up |
