6CY5

Crystal structure of Signal recognition particle receptor FtsY from Elizabethkingia anophelis in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-09-29
Detector	RIGAKU RAXIS
Wavelength(s)	0.9787
Spacegroup name	P 1
Unit cell lengths	49.520, 49.640, 69.710
Unit cell angles	101.18, 99.10, 105.58

Refinement procedure

Resolution	43.430 - 1.700
R-factor	0.158
Rwork	0.157
R-free	0.19360
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.430	43.430	1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge	0.048	0.037	0.243
Rmeas	0.052	0.038	0.287
Total number of observations	469119
Number of reflections	66029	752	4391
<I/σ(I)>	25.34	61.64	4.11
Completeness [%]	98.0	99.3	88.3
Redundancy	7.105	10.41	3.258
CC(1/2)	0.999	0.999	0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.7	290	tray 291448 H10: MCSG1: 0.1 M HEPES: NaOH, pH 7.5 25 % (w/v) PEG 3350 +4mM GTP/MgCl2: Cryo = 15%EG : ElanA.00983.a.B1 at 20 mg/ml, puck frp4-2