6CXL

anti-HIV-1 Fab 2G12 in complex with glycopeptide 10F5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	I 2 2 2
Unit cell lengths	137.641, 146.308, 148.213
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.808 - 3.586
R-factor	0.2201
Rwork	0.219
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.063
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.808	3.680
High resolution limit [Å]	3.586	3.586
Rmerge	0.296	1.000
Rmeas	0.316	1.000
Rpim	0.142	0.480
Number of reflections	17760	1170
<I/σ(I)>	5.8
Completeness [%]	99.8	99.9
Redundancy	8.5	8.6
CC(1/2)	0.920	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	0.2M lithium sulfate, 17.5% Peg400, 0.1M tris