6CVR
Human Aprataxin (Aptx) S242N bound to RNA-DNA, AMP and Zn product complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.616, 116.056, 117.563 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.390 - 1.880 |
R-factor | 0.1673 |
Rwork | 0.166 |
R-free | 0.18990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ndf |
RMSD bond length | 0.009 |
RMSD bond angle | 1.001 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.880 | 1.880 |
Number of reflections | 44982 | |
<I/σ(I)> | 17 | |
Completeness [%] | 97.3 | |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 200 mM ammonium formate, 20% w/v polyethylene glycol 3350 |