6CTZ
Structure of the GDP and kanamycin complex of APH(2")-IIia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.169, 58.982, 70.853 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.090 - 1.340 |
| R-factor | 0.147 |
| Rwork | 0.146 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tdw |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.086 | 1.360 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Number of reflections | 73255 | |
| <I/σ(I)> | 22.1 | 2.7 |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 14.3 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.25 M magnesium chloride, 0.1 M Tris-HCl, 20% PEG4000 |
