6CT5

PptT PAP(CoA) 8918 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	120
Detector technology	PIXEL
Collection date	2017-06-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	.93
Spacegroup name	C 1 2 1
Unit cell lengths	134.863, 63.408, 79.267
Unit cell angles	90.00, 123.15, 90.00

Refinement procedure

Resolution	39.586 - 1.759
R-factor	0.161648321635
Rwork	0.160
R-free	0.19806
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qjk 4qvh
RMSD bond length	0.012
RMSD bond angle	1.311
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.600	1.822
High resolution limit [Å]	1.759	1.759
Rmerge	0.111	0.574
Rmeas	0.126	0.655
Rpim	0.060	0.309
Number of reflections	55209	5471
<I/σ(I)>	16.6	2.27
Completeness [%]	99.1	98.54
Redundancy	4.5	4.6
CC(1/2)	0.912	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	291.15	Bis-Tris Propane pH 6.7, Magnesium Sulfate