6CST
Structure of human DNA polymerase kappa with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.579, 124.630, 165.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.140 - 2.000 |
| R-factor | 0.19923 |
| Rwork | 0.198 |
| R-free | 0.23416 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.532 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.140 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.550 |
| Rmeas | 0.079 | |
| Number of reflections | 93082 | 13313 |
| <I/σ(I)> | 12 | 2.8 |
| Completeness [%] | 98.3 | 97.4 |
| Redundancy | 4.5 | 4.7 |
| CC(1/2) | 0.998 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | 25% PEG 400 and 0.2M ammonium iodide |
