6CQD

Crystal structure of HPK1 in complex with ATP analogue (AMPPNP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	93
Detector technology	PIXEL
Collection date	2017-09-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	146.886, 51.882, 107.500
Unit cell angles	90.00, 131.03, 90.00

Refinement procedure

Resolution	46.987 - 2.120
R-factor	0.2059
Rwork	0.203
R-free	0.24980
RMSD bond length	0.005
RMSD bond angle	0.847
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.127
High resolution limit [Å]	2.120	2.120
Number of reflections	35007
<I/σ(I)>	8.8
Completeness [%]	99.9
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	0.1 M HEPES, pH 7.5, 12% PEG8000