6CPL
Crystal structure of DR11 presenting the gag293 epitope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 65.746, 65.746, 222.514 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.160 - 2.450 |
| R-factor | 0.225 |
| Rwork | 0.224 |
| R-free | 0.25290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mdo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.160 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rpim | 0.052 | 0.435 |
| Number of reflections | 20598 | 2328 |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | PEG 3350, Tris pH 8.5, Ethylene glycol |
