6CLU
Staphylococcus aureus Dihydropteroate Synthase (saDHPS) F17L E208K double mutant structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.979430 |
Spacegroup name | P 43 |
Unit cell lengths | 76.636, 76.636, 176.226 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.622 - 1.950 |
R-factor | 0.1893 |
Rwork | 0.182 |
R-free | 0.20360 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.001 |
RMSD bond angle | 0.371 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.622 | 1.990 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 684630 | |
<I/σ(I)> | 9.7 | |
Completeness [%] | 99.9 | |
Redundancy | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M Sodium Nitrate, and 20% PEG 3350 |