6CJX

Crystal structure of a Fc fragment LALA mutant (L234A, L235A) of human IgG1 (crystal form 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	49.000, 74.834, 145.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.753 - 2.440
R-factor	0.2184
Rwork	0.216
R-free	0.26350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ave
RMSD bond length	0.014
RMSD bond angle	1.656
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.480
High resolution limit [Å]	2.440	2.440
Rmerge	0.163	0.749
Rpim	0.071	0.399
Number of reflections	19011	710
<I/σ(I)>	20.5
Completeness [%]	91.7	69.7
Redundancy	5.3	3.9
CC(1/2)		0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	25% PEG 3,350 0.1 M Bus-Tris pH 6.5