6CJE
Crystal Structure of Mnk2-D228G in complex with Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 105.915, 105.915, 72.658 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.780 - 3.360 |
| R-factor | 0.21629 |
| Rwork | 0.212 |
| R-free | 0.31573 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hw7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.883 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.440 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Number of reflections | 6977 | |
| <I/σ(I)> | 4 | |
| Completeness [%] | 98.0 | |
| Redundancy | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 300.15 | 20-30% (w/v) polyacrylic acid 5100, 1-5% PEG 400, and 50 mM Hepes (pH 7.5) |
