6CJ5

Crystal Structure of Mnk2-D228G in Complex With Inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 32 2 1
Unit cell lengths	105.370, 105.370, 72.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.700 - 2.800
R-factor	0.2073
Rwork	0.204
R-free	0.28002
RMSD bond length	0.012
RMSD bond angle	1.891
Data reduction software	iMOSFLM
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.000	2.870
High resolution limit [Å]	2.800	2.800
Number of reflections	11166
<I/σ(I)>	14
Completeness [%]	99.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	300.15	20-30% (w/v) polyacrylic acid 5100, 1-5% PEG 400, and 50 mM Hepes (pH 7.5)