6CHT
HNF4alpha in complex with the corepressor EBP1 fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2015-05-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 139.721, 104.946, 139.563 |
| Unit cell angles | 90.00, 90.61, 90.00 |
Refinement procedure
| Resolution | 49.632 - 3.174 |
| R-factor | 0.4142 |
| Rwork | 0.262 |
| R-free | 0.27420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pzl |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.856 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.632 | 3.200 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.075 | 0.402 |
| Number of reflections | 48984 | |
| <I/σ(I)> | 9.3 | 2.7 |
| Completeness [%] | 92.5 | 76.2 |
| Redundancy | 3.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 293 | 0.1 m Bis-Tris, 26-28% MPD, 3-4% PEG8000, 20-50 mM Glycine and 10% glycerol |
