6CHB

Crystal structure of a natively-glycosylated BG505 SOSIP.664 HIV-1 Envelope Trimer in complex with the broadly-neutralizing antibodies BG18 and IOMA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	176.752, 176.752, 458.039
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.631 - 6.801
R-factor	0.3232
Rwork	0.318
R-free	0.41670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t3z 5ud9
RMSD bond length	0.003
RMSD bond angle	0.717
Data reduction software	XDS
Data scaling software	Aimless (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.700	39.700	7.020
High resolution limit [Å]	6.660	21.070	6.660
Rmerge	0.333	0.090	4.752
Rmeas	0.347	0.095	4.954
Rpim	0.097	0.029	1.365
Number of reflections	13894	430	1890
<I/σ(I)>	6.4
Completeness [%]	98.7	83.6	95
Redundancy	12.4	9.6	12.5
CC(1/2)	0.988	0.993	0.334

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	5% Tacismate pH 8.0, 15% PEG 3350