6CH9

Crystal structure of a natively-glycosylated B41 SOSIP.664 HIV-1 Envelope Trimer in complex with the broadly-neutralizing antibodies BG18 and 35O22

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	241.080, 241.080, 345.470
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.900 - 4.850
R-factor	0.285
Rwork	0.285
R-free	0.29200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t3z 5ud9 4toy
RMSD bond length	0.008
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.900	5.310
High resolution limit [Å]	4.850	4.850
Rmerge	0.259	5.063
Rmeas	0.273	5.351
Rpim	0.086	1.712
Total number of observations	183962
Number of reflections	18521	4397
<I/σ(I)>	5
Completeness [%]	99.8	100
Redundancy	9.9	9.6
CC(1/2)	0.996	0.347

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	5% Tacismate pH 8.0, 15% PEG 3350